Environmental Biotechnology
Online EB edition > 2017 Volume 1 > Article


Research

Treatment of simulated oil and gas production wastewater using Typha latifolia in a pilot-scale constructed wetland
Oludamilola Alalade, Jeremy Ferguson, John Pichtel

Pages: 1-10

DOI: 10.14799/ebms284

open PDF file


Abstract

During hydraulic fracturing (‘fracking’), large volumes of high-pressure, chemically-treated water are pumped into subsurface strata to free trapped petroleum and natural gas.  Chemically-enriched water, along with brine and groundwater, collectively termed oil and gas production water (PW), are eventually recovered from the well.  PW poses environmental and health risks; however, it can be reused if potentially hazardous constituents are removed.  A two-stage pilot-scale constructed wetland containing cattail (Typha latifolia) was tested for treatment of synthetic PW.  After 49 days, PW pH increased from 4.2 to 7.0, and electrical conductivity decreased from 22,100 to 3,300µS·cm-1.  Typha shoots had bioconcentration factors for Pb ranging from 2.8 in (Stage 1 of constructed wetland) to 8.0 (Stage 2).  Transfer factors for Pb were 0.67 (Stage 1) and 1.37 (Stage 2).  These results indicate that Typha may be effective for Pb removal from PWs.  The present study may be of practical value to oil and gas production companies that plan to recycle or properly dispose of large quantities of oil and gas production wastewater.

.


References

 

ALL Consulting. 2009. Ground Water Protection Council, Modern Shale Gas Development in the United States: A Primer. U.S. Department of Energy, Office of Fossil Energy, Washington, DC.

Allen, S.E., M.H. Grimshaw, J.A. Parkinson, C. Quarmby. 1974. Chemical Analysis of Ecological Materials (ed. S.E. Allen). 386p. Blackwell Scientific Publications, Oxford London, Edinburg, Melbourne.

APHA (American Public Health Association). 2017. Metals by flame atomic absorption spectrometry, Method 3111. Standard Methods for the Examination of Water and Wastewater. Washington, DC.

Aqwatec. 2015. Produced water beneficial use case studies, Produced Water Treatment and Beneficial Use Information Center. http://aqwatec.mines.edu/produced water/assessbu/case/, accessed January 27, 2017.

Arroyo, P., G. Ansola, E. de Luis. 2010. Effectiveness of a full-scale constructed wetland for the removal of metals from domestic wastewater. Water Air Soil Pollution 210: 473-481.
https://doi.org/10.1007/s11270-009-0272-9

Baker, A.J.M. 1981. Accumulators and excluders—strategies in the response of plants to heavy metals. Journal of Plant Nutrition 3: 643–654.
https://doi.org/10.1080/01904168109362867

Baker, A.J.M., R.D. Reeves, A.S.M. Hajar. 1994. Heavy metal accumulation and tolerance in British populations of the metallophyte Thlaspi caerulescens J.&C Presl (Brassicaceae). New Phytologist 127: 61–68.
https://doi.org/10.1111/j.1469-8137.1994.tb04259.x

Bandaruk, T., S. Waara. 2014. Metal, metalloid and sulphur sequestration in a constructed wetland for treatment of landfill leachate during 2003-2012. Conference Proceedings for Linneus-Eco Tech. Nov. 24-26, 2014, Kalmar, Sweden.

Benko, K.L., J.E. Drewes. 2008. Produced water in the western United States: Geographical distribution, occurrence, and composition. Environmental Engineering Science 25: 239-246.
https://doi.org/10.1089/ees.2007.0026

Bergman, A., J.J. Heindel, S. Jobling, K.A. Kidd, R.T. Zoeller. 2013. State of the science of endocrine disrupting chemicals. World Health Organization. http://www.who.int/ceh/publications/endocrine/en/, accessed January 8, 2017.

Black, C.A. 1965. Nitrogen-Total. Methods of Soil Analysis. Chemical And Microbiological Properties. American Society of Agronomy, Madison, WI.

Blaylock, M.J., J.W. Huang. 2000. Phytoextraction of metals. In: Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment (ed. I. Raskin and B.D. Ensley), pp. 53–71. New York, John Wiley & Sons.

Boyd, C.E. 1978. Chemical composition of wetland plants. In: Freshwater Wetlands. Ecological Processes and Management Potential (ed. R.E. Good, D.F. Whigham and R.L. Simpson), pp. 155–167. Academic Press, NY.

Branquinho, C., H.C. Serrano, M.J. Pinto, M.A. Martins-Loucao. 2007. Revisiting the plant hyperaccumulation criteria to rare plants and earth abundant elements. Environmental Pollution 14: 437-443.
https://doi.org/10.1016/j.envpol.2006.06.034

Bray, R.H., L.T. Kurtz.1945. Determination of total, organic, and available forms of phosphorus in soils. Soil Science 59: 39-45.
https://doi.org/10.1097/00010694-194501000-00006

Brisson, J., F. Chazarenc. 2009. Maximizing pollutant removal in constructed wetlands: Should we pay more attention to macrophyte species selection? Science of the Total Environment 407: 3923–3930.
https://doi.org/10.1016/j.scitotenv.2008.05.047

Burau, R.G. 1982. Lead. In: Methods of Soil Analysis. Part 2. (ed. A.L. Page, R.H. Miller and D.R. Keeney), pp. 347-365. American Society of Agronomy, Madison, WI.

Burton, G.A., K.J. Scott. 1992. Assessing contaminated aquatic sediments. Environmental Science and Technology 26: 2068–2075.
https://doi.org/10.1021/es00035a002

Carranza-Alvarez, C., A.J. Alonso-Castro, M.C. Alfaro-De La Torre, R.F. Garcia-De La Cruz. 2008. Accumulation and distribution of heavy metals in Scirpus americanus and Typha latifolia from an artificial lagoon in San Luis Potosi, Mexico. Water Air Soil Pollution 188: 297–309.
https://doi.org/10.1007/s11270-007-9545-3

Chaudhuri, P., B. Nath, G. Birch. 2014. Accumulation of trace metals in grey mangrove Avicennia marina fine nutritive roots: The role of rhizosphere processes. Marine Pollution Bulletin 79: 284-292.
https://doi.org/10.1016/j.marpolbul.2013.11.024

Chung, A.K.C., Y. Wu, N.F.Y. Tam, N.F. Wong. 2008. Nitrogen and phosphate mass balance in a sub-surface flow constructed wetland for treating municipal wastewater. Ecological Engineering 32: 81–89.
https://doi.org/10.1016/j.ecoleng.2007.09.007

Clark, C.E., J.A. Veil. 2009. Produced water volumes and management practices in the United States. Environmental Science Division, Argonne National Laboratory. 64 p. ANL/EVS/R-09/1.
https://doi.org/10.2172/1007397

Coon, W.F., J.M. Bernard, F.K. Seischab. 2000. Effects of a cattail wetland on water quality of Irondequoit Creek near Rochester, New York. Water-Resources Investigations Report 2000-4032. https://pubs.usgs.gov/wri/2000/4032/wri20004032.pdf, accessed April 19, 2017.

 Deng, H., Z.H. Ye, M.H. Wong. 2004. Accumulation of lead, zinc, copper and cadmium by 12 wetland plant species thriving in metal-contaminated sites in China. Environmental Pollution 132: 29–40.
https://doi.org/10.1016/j.envpol.2004.03.030
 

Dunbabin, J.S., K.H. Bowmer. 1992. Potential use of constructed wetlands for treatment of industrial wastewaters containing metals. The Science of the Total Environment 111: 151–168.
https://doi.org/10.1016/0048-9697(92)90353-T

Earthworks. 2015. Hydraulic Fracturing 101. Hydraulic fracturing—What it is. https://www.earthworksaction.org/issues/detail/hydraulic_fracturing_101#.WG6gxH0aJ-Y, accessed January 27, 2017.

Fontenot, B.E., L.R. Hunt, Z.L. Hildenbrand, D.D. Carlton, Jr., H. Oka, J.L. Hopkins, A. Osorio, B. Bjorndal, Q.H. Hu, K.A. Schug. 2013. An evaluation of water quality in private drinking water wells near natural gas extraction sites in the Barnett Shale formation. Environmental Science & Technology 47: 10032–10040.
https://doi.org/10.1021/es4011724

FracFocus. 2015. Hydraulic fracturing the process. https://fracfocus.org/hydraulic- fracturing-how-it- works/hydraulicfracturing-process, accessed January 12, 2017.

Fritioff, A., M. Greger. 2006. Uptake and distribution of Zn, Cu, Cd and Pb in an aquatic plant Potamogeton natans. Chemosphere 63: 220–227.
https://doi.org/10.1016/j.chemosphere.2005.08.018

Gersberg, R.M., S.R. Lyon, B.V. Elkins, C.R. Goldman. 1984. The removal of heavy metals by artificial wetlands. In: Proceedings of the Conference on the Future of Water Use, pp. 639–648.

Gottschall, N., C. Boutin, A. Crolla, C. Kinsley, P. Champagne. 2007. The role of plants in the removal of nutrients at a constructed wetland treating agricultural (dairy) wastewater, Ontario, Canada. Ecological Engineering 29: 154–163.
https://doi.org/10.1016/j.ecoleng.2006.06.004

Igunnu, E.T., G.Z. Chen. 2014. Produced water treatment technologies. International Journal of Low-Carbon Technologies 9: 157–177.
https://doi.org/10.1093/ijlct/cts049

Jesus, J.M., C.C. Calheiros, P.M. Castro, M.T. Borges. 2014. Feasibility of Typha latifolia for high salinity effluent treatment in constructed wetlands for integration in resource management systems. International Journal of Phytoremediation 16: 334-346.
https://doi.org/10.1080/15226514.2013.773284

Kadlec, R., S. Roy, R. Munson, S. Charlton, W. Brownlie. 2010. Water quality performance of treatment wetlands in the Imperial Valley, California. Ecological Engineering 36: 1093–1107.
https://doi.org/10.1016/j.ecoleng.2010.04.028

Kaewtubtim, P.W. Meeinkuirt, S. Seepom, J. Pichtel. 2016. Heavy metal phytoremediation potential of mangrove plant species of Pattani Bay, Thailand. Applied Ecology 14: 367-382.
https://doi.org/10.15666/aeer/1401_367382

Kassotis, C.D., D.E. Tillitt, C.-H. Lin, J.A. McElroy, S.C. Nagel. 2015. Endocrine-disrupting chemicals and oil and natural gas operations: potential environmental contamination and recommendations to assess complex environmental mixtures. Environmental Health Perspectives https://ehp.niehs.nih.gov/wp content/uploads/advpub/2015/8/ehp.1409535.acco.pdf, accessed January 27, 2017.

Khan, S., I. Ahmad, M.T. Shah, S. Rehman, A. Khaliq. 2009. Use of constructed wetland for the removal of heavy metals from industrial wastewater. Journal of Environmental Management 90: 3451–3457.
https://doi.org/10.1016/j.jenvman.2009.05.026

Khatib, Z., P. Verbeek. 2003. Water to value-produced water management for sustainable field development of mature and green fields. Journal of Petroleum Technology: 26-28.
https://doi.org/10.2118/0103-0026-JPT

Kim, K.R., G. Owens, R. Naidu, K.H. Kim. 2008. Influence of vetiver grass (Vetiveria zizanioides) on rhizosphere chemistry in long-term contaminated soils. Korean Journal of Soil Science and Fertilizer 41: 55–64.

Klucakova, M. 2012. Comparative study of binding behaviour of Cu(II) with humic acid and simple organic compounds by ultrasound spectrometry. The Open Colloid Science Journal 5: 5-12.
https://doi.org/10.2174/1876530001205010005

Knox, A.S., E.A. Nelson, N.V. Halverson, J.B. Gladden. 2010. Long-term performance of a constructed wetland for metal removal. Soil and Sediment Contamination 19: 667–685.
https://doi.org/10.1080/15320383.2010.515628

Kröpfelová, L., J. Vymazal, J. Švehla, J. Štíchová. 2009. Removal of trace elements in three horizontal sub-surface flow constructed wetlands in the Czech Republic. Environmental Pollution 157: 1186–1194.
https://doi.org/10.1016/j.envpol.2008.12.003

Lacerda, L.D., M.A. Fernandez, C.F. Calazans, K.F. Tanizaki. 1992. Bioavailability of heavy metals in sediments of two coastal lagoons in Rio de Janeiro, Brazil. Hydrobiologia 228: 65-70.
https://doi.org/10.1007/BF00006477

Lacerda, L.D., C.E.V. Carvalho, K.F. Tanizaki, A.R.C. Ovalle, C.E. Rezende. 1993. The biogeochemistry and trace metals distribution of mangrove rhizospheres. Biotropica 25: 252-257.
https://doi.org/10.2307/2388783

Lesage, E. 2006. Behaviour of heavy metals in constructed treatment wetlands. PhD thesis. 247 p. Department of Applied Analytical and Physical Chemistry, Ghent University, Belgium.

Lasat, M.M., N.S. Pence, D.F. Garvin, S.D. Ebbs, L.V. Kochian. 2000. Molecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens. Journal of Experimental Botany 51: 71–79.
https://doi.org/10.1093/jxb/51.342.71

Machado, W., B.B. Gueiros, S.D. Lisboa-Filho, L.D. Lacerdo. 2005. Trace metals in mangrove seedlings: role of iron plaque formation. Wetlands Ecology and Management 13: 199-206.
https://doi.org/10.1007/s11273-004-9568-0

Maguire-Boyle, S.J., A.R. Barron. 2014. Organic compounds in produced waters from shale gas wells. Environmental Science Processes & Impacts 16: 2237-2248.
https://doi.org/10.1039/C4EM00376D

Maine, M.A., N. Sune, H. Hadad, G. Sanchez, C. Bonetto. 2006. Nutrient and metal removal in a constructed wetland for wastewater treatment from a metallurgic industry. Ecological Engineering 26: 341–347.
https://doi.org/10.1016/j.ecoleng.2005.12.004

Marcellus. 2010. List of 78 Chemicals Used in Hydraulic Fracturing Fluid in Pennsylvania. http://marcellusdrilling.com/2010/06/list-of-78-chemicals-used-in-hydraulic-fracturing-fluid-in-pennsylvania, accessed August 23, 2016.

Marchand, C., M. Allenbach, E. Lallier-Vergès. 2011. Relationships between heavy metals distribution and organic matter cycling in mangrove sediments (Conception Bay, New Caledonia). Geoderma 160: 444–456.
https://doi.org/10.1016/j.geoderma.2010.10.015

Markert, B. 1992. Presence and significance of naturally occurring chemical elements of the periodic system in the plant organism and consequences for future investigations on inorganic environmental chemistry in ecosystems. Vegetatio 103: 1–30.

McNaughton, S.J., T.C. Folsom, T. Lee, F. Park, C. Price, D. Roeder, J. Schmitz, C. Stockwell. 1974. Heavy metal tolerance in Typha latifolia without the evolution of tolerant races. Ecology 55: 1163–1165.
https://doi.org/10.2307/1940369

Meeinkuirt, W., M. Kruatrachue, J. Pichtel, T. Phusantisampan, P. Saengwilai. 2016. Influence of organic amendments on phytostabilization of Cd-contaminated soil by Eucalyptus camaldulensis. ScienceAsia 42: 83-91.
https://doi.org/10.2306/scienceasia1513-1874.2016.42.083

Meeinkuirt, W., P. Pokethitiyook, M. Kruatrachue, P. Tanhan, R. Chaiyarat R. 2012. Phytostabilization of lead by various tree species using pot and field trial experiments. International Journal of Phytoremediation 14: 925–938.
https://doi.org/10.1080/15226514.2011.636403

Miretzky, P., A. Saralegui, A.F. Cirelli. 2004. Aquatic macrophytes potential for the simultaneous removal of heavy metals (Buenos Aires, Argentina). Chemosphere 57: 997–1005.
https://doi.org/10.1016/j.chemosphere.2004.07.024

Mojiri, A., H.A. Aziz, M.A. Zahed, S.Q. Aziz, M.R.B. Selamat. 2013. Phytoremediation of heavy metals from urban waste Leachate by southern cattail (Typha domingensis). International Journal of Scientific Research in Environmental Sciences 1: 63-70.
https://doi.org/10.12983/ijsres-2013-p063-070

Moshiri, G.A. 1993. Constructed Wetlands for Water Quality Improvement. 633 p. CRC Press, Boca Raton, FL.

Mungur, A.S., R.B.E. Shutes, D.M. Revitt, M.A. House. 1997. An assessment of metal removal by a laboratory scale wetland. Water Science and Technology 35: 125–133.

Munzuroglu, O., H. Geckil. 2002. Effects of metals on seed germination, root elongation, and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus. Archives of Environmental Contamination and Toxicology 43: 203-213.
https://doi.org/10.1007/s00244-002-1116-4

Murray Gulde, C., J.E. Heatley, T. Karanfil, J.H. Rodgers Jr., J.E. Myers. 2003. Performance of a hybrid reverse osmosis-constructed wetland treatment system for brackish oil field produced water. Water Research 37: 705-713.
https://doi.org/10.1016/S0043-1354(02)00353-6

Murray, H., K. Thompson, S.M. Macfie. 2009. Site- and species-specific patterns of metal bioavailability in edible plants. Botany 87: 702-711.
https://doi.org/10.1139/B09-019

NPC (National Petroleum Council). 2011. Management of Produced Water from Oil and Gas Wells, Paper 2–17. NPC North American Resource Development Study. https://www.npc.org/Prudent Development-Topic Papers/2-17, Management of Produced Water Paper.pdf. accessed January 27, 2017.

O'Rourke, D., S. Connolly. 2003. Just oil? The distribution of environmental and social impacts of oil production and consumption. Annual Review of Environmental Resources 28: 587-617.
https://doi.org/10.1146/annurev.energy.28.050302.105617

Osmolovskaya, N.G., V.V. Kurilenko. 2001. Biogeochemical aspects of heavy metals phytoindication in urban aquatic systems. In: Biogeochemical Processes and Cycling of Elements in the Environment (ed. J. Weber), pp. 217–218. Polish Society of Humic Substances, Wroclaw.

Otero, A., S. Fiol, J. Antelo, F. Arce. 2015. Studying and modeling the effect of organic matter on the sorption of inorganic ions on goethite. Goldschmidt 2015. Abstracts, http://goldschmidt.info/2015/uploads/abstracts/finalPDFs/2365.pdf accessed December 14, 2016.

Otero, X.L., T.O. Ferreira, P. Vida-Torrado, F. Macias. 2006. Spatial variation in pore water geochemistry in a mangrove system (Pai Matos island, Cananeia-Brazil). Applied Geochemistry 21: 2171-2186.
https://doi.org/10.1016/j.apgeochem.2006.07.012

Panich-Pat, T., P. Pokethitiyook, M. Kruatrachue, E.S. Upatham, P. Srinives, G.R. Lanza. 2004. Removal of lead from contaminated soils by Typha angustifolia. Water, Air and Soil Pollution 155: 159–171.
https://doi.org/10.1023/B:WATE.0000026523.96599.6b

Pantip, K., N. Suwanchai. 2004. Constructed treatment wetland: a study of eight plant species under saline conditions. http://dspace.library.tu.ac.th/handle/3517/1435, accessed January 27, 2017.

Phaenark, C., P. Pokethitiyook, M. Kruatrachue, C. Ngernsansaruay. 2009. Cd and Zn accumulation in plants from the Padaeng zinc mine area. International Journal of Phytoremediation 11:479–495.
https://doi.org/10.1080/15226510802656243

Pichtel, J. 2016. Oil and gas production wastewater: Soil contamination and pollution prevention. Applied and Environmental Soil Science. p. 1-24. downloads.hindawi.com/journals/aess/aip/2707989.pdf, accessed December 14, 2016.
https://doi.org/10.1155/2016/2707989

Pip, E., J. Stepaniuk. 1992. Cadmium, copper and lead in sediments. Archive fur Hydrobiologie 124: 337–355.

Rezvani, M., F. Zaefarian. 2011. Bioaccumulation and translocation factors of cadmium and lead in Aeluropus littoralis. Australian Journal Agricultural Engineering 2: 114-119.

Romheld, V., H. Marschner. 1986. Mobilization of iron in the rhizosphere of different plant species. Advances in Plant Nutrition 2: 155–204.

Sasmaz, A., E. Obek, H. Hasar. 2008. The accumulation of heavy metals in Typha latifolia L. grown in a stream carrying secondary effluent. Ecological Engineering 33: 278-284.
https://doi.org/10.1016/j.ecoleng.2008.05.006

Skinner, K., N. Wright, E. Porter-Goff. 2007. Mercury uptake and accumulation by four species of aquatic plants. Environmental Pollution 145: 234–237.
https://doi.org/10.1016/j.envpol.2006.03.017

Sparks, D.L. 1996. Methods of soil analysis. Part 3. Chemical methods. In: Soil Science Society of America (ed. J. Bartels, J.M. Bigham), pp. 555-574. Madison WI.

Svengsouck, L.J., W.J. Mitsch. 2000. Dynamics of mixtures of Typha latifolia and Schoenoplectus tabernaemontani in nutrient-enrichment wetland experiments. The American Midland Naturalist 145: 309-324.
https://doi.org/10.1674/0003-0031(2001)145[0309:DOMOTL]2.0.CO;2

USDA-NRCS (US Department of Agriculture-Natural Resources Conservation Service). 2015. A Handbook of Constructed Wetlands https://www.epa.gov/sites/production/files/2015-10/documents/constructed-wetlands-handbook.pdf. pp 6, accessed December 14, 2016.<

USDA-NRCS. 2017. Soil Survey for Delaware County, Indiana. https://websoilsurvey.sc.egov.usda.gov/App/WebSoilSurvey.aspx., accessed December 01, 2017.

USEPA (United States Environmental Protection Agency). 2004. Chapter 2: Guidelines for water reuse. EPA/625/R-04/108, Washington, D.C.

Veil, A., M.G. Ruder, D. Elcock, R.J. Redweik, Jr. 2004. A white paper describing produced water from production of crude oil, natural gas, and coal bed methane. 87 p. United States Department of Energy, National Energy Technology Laboratory, Contract W-31-109-Eng-38.

Vymazal, J. 2005. Horizontal sub-surface flow and hybrid constructed wetlands systems for wastewater treatment. Ecological Engineering 25: 478–490.
https://doi.org/10.1016/j.ecoleng.2005.07.010

Walker, D.J., S. Hurl. 2002. The reduction of heavy metals in a stormwater wetland. Ecological Engineering 18: 407–414.
https://doi.org/10.1016/S0925-8574(01)00101-X

Walkley, A., I.A. Black. 1934. An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science 37: 29-37.
https://doi.org/10.1097/00010694-193401000-00003

Warner, N.R., R.B. Jackson, T.H. Darrah, S.G. Osborn, A. Down, K. Zhao, A. White, A. Vengosh. 2012. Geochemical evidence for possible natural migration of Marcellus formation brine to shallow aquifers in Pennsylvania. Proceedings of the National Academy of Sciences of the United States of America 109: 11961–11966.
https://doi.org/10.1073/pnas.1121181109

Wei, C.Y., T.B. Chen, Z.C. Huang. 2002. Cretan bake (Pteris cretica L.): an arsenic-accumulating plant. Acta Ecologica Sinica 22: 777–782.

Weis, J.S., P. Weis. 2004. Metal uptake, transport and release by wetland plants: Implications for phytoremediation and restoration review. Environment International 30: 685–700.
https://doi.org/10.1016/j.envint.2003.11.002

Woo, I., J.B. Zedler. 2002. Can nutrients alone shift a sedge meadow towards dominance by the invasive Typha X Glauca? Wetlands 22: 509-521.
https://doi.org/10.1672/0277-5212(2002)022[0509:CNASAS]2.0.CO;2

Ye, Z.H., A.J.M. Baker, M.H. Wong, A.J. Willis. 1997. Zinc, lead and cadmium tolerance, uptake and accumulation by Typha latifolia. New Phytologist 136: 469–480.
https://doi.org/10.1046/j.1469-8137.1997.00759.x

Zhao, F.J., E. Lombi, S.P. Mc Grath. 2003. Assessing the potential for zinc and cadmium phytoremediation with the hyperaccumulator Thlaspi caerulescens. Plant and Soil 249: 37–43.
https://doi.org/10.1023/A:1022530217289

Zhou, L.X., J.W.C. Wong. 2001. Effect of dissolved organic matter from sludge and sludge compost on soil copper sorption. Journal of Environmental Quality 30: 878-883.
https://doi.org/10.2134/jeq2001.303878x

Zu, Y.Q., J. Li, Y. Chen, H.Y. Chen, L. Qin, C. Schvartz. 2005. Hyperaccumulation of Pb, Zn and Cd in herbaceous grown on lead-zinc mining area in Yunnan, China. Environment International 31: 755–762.
https://doi.org/10.1016/j.envint.2005.02.004

 

  © ChemProf 2009