Environmental Biotechnology
Online EB edition > 2016 Volume 2 > Article


Plant growth promoting properties of Serratia fonticola ART-8 and Pseudomonas putida ART-9 and their effect on the growth of spring wheat (Triticum aestivum L.)
Sebastian W. Przemieniecki, Tomasz P. Kurowski, Karol Kotlarz, Krzysztof Krawczyk, Marta Damszel, Anna Karwowska

Pages: 35-39

DOI: 10.14799/ebms263

open PDF file


Two bacterial strains, Serratia fonticola ART-8 and Pseudomonas putida ART-9, were isolated from soil sown with rye in a monoculture. Although the S. fonticola strain produced more chitinase than the P. putida strain, P. putida produced more cellulase, lipase, HCN, and fluorescent siderophores. P. putida also solubilized more phosphate, and was the only strain to produce pyoverdine. Neither bacteria produced indoleacetic acid (IAA), nor did they produce siderophores on the CAS medium. Neither of the strains was highly effective at inhibiting Fusarium culmorum (~5%) or F. oxysporum (~24%). Wheat plants inoculated with these bacterial strains had higher (5.7-10.0%) thousand kernel weight and there appeared to be a positive association between thousand kernel weight and ear length..


Ahemad, M., M.S. Khan. 2011. Assessment of plant growth promoting activities of rhizobacterium Pseudomonas putida under insecticide stress. Microbiology Journal 1: 54–64.

Ahemad, M., M.S. Khan. 2012. Effect of fungicides on plant growth promoting activities of phosphate solubilizing Pseudomonas putida isolated from mustard (Brassica compestris) rhizosphere. Chemosphere 86: 945–950.

Ahemad, M., M. Kibret. 2014. Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University – Science 26. http://dx.doi.org/10.1016/j.jksus.2013.05.001

Altschul, S., W. Gish, W. Miller, E. Myers, D. Lipman. 1990. Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.

Aljorayid, A., R. Viau, L. Castellino, R.L. Jump. 2016. Serratia fonticola, pathogen or bystander? A case series and review of the literature. IDCases 5: 6–8.

Ashwini, N., S. Srividya. 2014. Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1.3 Biotech 4. http://link.springer.com/article/10.1007%2Fs13205-013-0134-4

Beneduzi, A., A. Ambrosini, L.M.P. Passaglia. 2012. Plant growth promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology 34 (4 suppl.): 1044–1051.

Bhattacharyya, P.N., D.K. Jha. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28: 1327–1350.

Chen, Y.P., P.D. Rekha, A.B. Arun, F.T. Shen, W.-A. Lai, C.C. Young. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34: 33–41.

Ghodsalavi, B., M. Ahmadzadeh, M. Soleimani, P.B. Madloo, R. Taghizad-Farid. 2013. Isolation and characterization of rhizobacteria and their effects on root extracts of Valeriana officinalis. Australian Journal of Crop Science 7: 338–344.

Hagedorn, C., J.G. Holt. 1975. Differentiation of Arthrobacter soil isolated and named strains from other bacteria by reactions on dye containing media. Canadian Journal of Microbiology 21: 688-693.

Hankin, L., M. Zucker, D.C. Sands. 1971. Improved solid medium for the detection and enumeration of pectolytic bacteria. Applied Microbiology 22: 205–209.

Hinsinger, P., A.G. Bengough, D. Vetterlein, I.M. Young. 2009. Rhizosphere: biophysics, biogeochemistry and ecological relevance. Plant Soil 321: 117–152.

Jacobsen, C.S, M.H. Hjelmso. 2014. Agricultural soils, pesticides and microbial diversity. Current Opinion in Biotechnology 27: 15–20.

Lane, D.J. 1991. 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics (ed. E. Stackebrandt, M. Goodfellow, pp. 115-175. Willey, New York.

Meyer, J.M. 2000. Pyoverdines: Pigments, siderophores and potential taxonomic markers of fluorescent Pseudomonas species. Archives of Microbiology 174: 135–142.

Milagres, A.M., A. Machuca, D. Napoleao. 1999. Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. Journal of Microbiological Methods 37: 1–6.

Nautiyal, S.C. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170: 265–270.

Przemieniecki, S.W., T.P. Kurowski, K. Korzekwa, A. Karwowska. 2014. The effect of psychrotrophic bacteria isolated from the root zone of winter wheat on selected biotic and abiotic factors. Journal of Plant Protection Research 54: 407–413.

Przemieniecki, S.W., T.P. Kurowski, A. Karwowska. 2015. Plant growth promoting potential of Pseudomonas sp. SP0113 isolated from potable water from a closed water well. Archives of Biological Sciences 67: 663–673.

Roberts, W.K., C.P. Selitrennikoff. 1988. Plant and bacterial chitinases differ in antifungal activity. Journal of General Microbiology 134: 169-176.

Tasic, S., D. Obradovic, I. Tasic. 2013. Characterization of Serratia fonticola, an opportunistic pathogen isolated from drinking water. Archives of Biological Sciences 65: 899–904.

van der Heijden, M.G.A., R.D. Bardgett, N.M. van Straalen. 2008. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecology Letters 11: 296-310.

  © ChemProf 2009