Environmental Biotechnology
Online EB edition > 2015 Volume 1 > Article


Research

Application of by-products and waste in the synthesis of nanosilver particles
Beata Trepanowska, Michał K. Łuczyński, Sławomir Kulesza, Marek Adamczak

Pages: 14-19

DOI: 10.14799/ebms257

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Abstract

Extracts from strawberry and raspberry leaves, carrot pomace, and spent grains, were tested as bioreducing agents for the synthesis of nanosilver particles (AgNP). Based on UV vis spectra, the leaf extracts produced the most AgNP, carrot pomace was less effective, and spent grains did not produce AgNP. The dynamic light scattering method revealed that AgNP ranged from 1 to 92nm in size, and that over 80% of the particles were about 10nm. Energy dispersive X ray spectroscopy showed that elements that typically stabilize nanoparticles were present. The well diffusion method (nutrient agar medium) indicated that AgNP synthesized with raspberry leaf extract exerted strong bacteriostatic and bactericidal activity against Gram negative bacteria and weaker activity against Gram positive bacteria. Although further analysis is needed to determine the mechanism of their synthesis, the results of this study show that plant extract based synthesis can produce nanoparticles with controlled size and morphology.


References

Banerjee, P., M. Satapathy, A. Mukhopahayay, P. Das. 2014. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresources and Bioprocessing 1: 3
http://dx.doi.org/10.1186/s40643-014-0003-y
Chung, P.Y., Y.S. Toh. 2014. Anti-biofilm agents: recent breakthrough against multi-drug resistant Staphylococcus aureus. Pathogens and Disease 70: 231-239.
http://dx.doi.org/10.1111/2049-632X.12141
Dare, E.O., C.O. Oseghale, A.H. Labulo, E.T. Adesuji, E.E. Elemike, J.C. Onwuka, J.T. Bamgbose. 2015. Green synthesis and growth kinetics of nanosilver under bio-diversified plant extracts influence. Journal of Nanostructure in Chemistry 5: 85-94.
http://dx.doi.org/10.1007/s40097-014-0139-5
Dhas, S.P., S.P. John, A. Mukherjee, N. Chandrasekaran. 2014. Autocatalytic growth of biofunctionalized antibacterial silver nanoparticles. Biotechnology and Applied Biochemistry 61: 322-332.
http://dx.doi.org/10.1002/bab.1161
Elbeshehy, E.K.F., A.M. Elazzazy, G. Aggelis. 2015. Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticles characterization and their activity against Bean Yellow Mosaic Virus and human pathogens. Frontiers in Microbiology 6: 453.
http://dx.doi.org/10.3389/fmicb.2015.00453
Franci, G., A. Falanga, S. Galdiero, L. Palomba, M. Rai, G. Morelli, M. Galdiero. 2015. Silver nanoparticles as potential antibacterial agents. Molecules 20: 8856-8874.
http://dx.doi.org/10.3390/molecules20058856
Gurunathan, S., J. Han, D.-N. Kwon, J.-H. Kim. 2014. Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Research Letters 9: 373.
http://dx.doi.org/10.1186/1556-276X-9-373
Königs, A.M., H.-C. Flemming, J. Wingender. 2015. Nanosilver induces a nonculturable but metabolically active state in Pseudomonas aeruginosa. Frontiers in Microbiology 6: 395.
http://dx.doi.org/10.3389/fmicb.2015.00395
Miller, K.P., L. Wang, Y.P. Chen, P.J. Pellechia, B.C. Benicewicz, A.W. Decho. 2015. Engineering nanoparticles to silence bacterial communication. Frontiers in Microbiology 6: 189.
http://dx.doi.org/10.3389/fmicb.2015.00189
Mittal, A.K., Y. Chisti, U.C. Banerjee. 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances 31: 346-356.
http://dx.doi.org/10.1016/j.biotechadv.2013.01.003
Oskam, G., Z. Hu, R.L. Penn, N. Pesika, P.C Searson. 2002. Coarsening of metal oxide nanoparticles. Physical Review E 66: 1.
http://dx.doi.org/10.1103/PhysRevE.66.011403
Park, Y. 2014. New paradigm shift for the green synthesis of antibacterial silver nanoparticles utilizing plant extracts. Toxicological Research 30: 169-178.
http://dx.doi.org/10.5487/TR.2014.30.3.169
Ramar, M., B. Manikandan, P.N. Marimuthu, T. Raman, A. Mahalingam, P. Subramanian, S. Karthick, A. Munusamy. 2015. Synthesis of silver nanoparticles using Solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line MCF 7. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 40: 223-228.
http://dx.doi.org/10.1016/j.saa.2014.12.060
Sintubin, L., W. Verstraete, N. Boon. 2012. Biologically produced nanosilver: Current state and future perspectives. Biotechnology and Bioengineering 109: 2422-2436.
http://dx.doi.org/10.1002/bit.24570
Vilchis-Nestor, A.R., V. Sánchez-Mendieta, M.A. Camacho-López, R.M. Gómez-Espinosa, M.A. Camacho-López, J.A. Arenas-Alatorre. 2008. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Materials Letters 62: 3103-3105.
http://dx.doi.org/10.1016/j.matlet.2008.01.138
Wu, D., W. Fan, A. Kishen, J.L. Gutmann, B. Fan. 2014. Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm. Journal of Endodontics 40: 285-290.
http://dx.doi.org/10.1016/j.joen.2013.08.022

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